11 Mb) than the PGM chips, but the read lengths (200–500 bp), run time (4–5 h), and accuracy (99%) are similar [54, 72] . Sample preparation for the generation .... identification SNPs (IISNPs) [11] (four SNPs overlap between these two panels: ... programme was 2 min at 99 8C, 18 cycles of 15 s at 99 8C and 4 min at 60 8C followed by ... to emPCR and subsequent sequencing on two Ion 314 Chips. Fig.
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